Fig 1: Effect of CYC1 on SCC9 cells. At 48 h after cell transfection with lncRNA C5orf66-AS1-siRNA or lncRNA C5orf66-AS1-siRNA + CYC1-siRNA, the (A) proliferation, (B) apoptosis, (C) migration and (D) invasion of SCC9 cells were determined using an MTT assay, flow cytometry, wound healing assay and transwell assay, respectively. Wound closure was quantified and the number of invasive cells was counted. **P<0.01 vs. control groups. Magnification, ×100 (migration) and ×200 (invasion). lncRNA, long non-coding RNAs; C5orf66-AS1, C5orf66 antisense RNA 1; siRNA, small interfering RNA; NC, negative control; CYC1, cytochrome c1.
Fig 2: CYC1 was negatively regulated by lncRNA C5orf66-AS1. CYC1 mRNA expression in (A) OSCC and adjacent normal tissues, and in (B) OSCC cell line SCC9 and normal human oral keratinocyte cells was detected using reverse transcription-quantitative polymerase chain reaction. CYC1 mRNA and protein levels were also detected in cells transfected with (C and E) lncRNA C5orf66-AS1-plasmid or (D and F) lncRNA C5orf66-AS1-siRNA. **P<0.01 vs. control groups. lncRNA, long non-coding RNAs; C5orf66-AS1, C5orf66 antisense RNA 1; siRNA, small interfering RNA; NC, negative control; CYC1, cytochrome c1.
Supplier Page from Abcam for Anti-CYC1 antibody